picrosirius red staining solution  (Beijing Solarbio Science)

Journal: Nutrients

Article Title: Promoting Glutathione Synthesis: A Possibility for Treating Cardiomyopathy Induced by a Maternal Western Diet

doi: 10.3390/nu16152520

Figure Lengend Snippet: Myocardial interstitial fibrosis in offspring on postnatal day 7. ( A ) Representative images of Masson’s trichrome-stained transverse cardiac sections in neonatal offspring; areas positive for collagen were stained blue. ( B ) Representative images of ventricular mid-cardiac sections stained with picrosirius red and polarized light detection in offspring: areas positive for collagen were stained red in picrosirius red staining, areas positive for collagen type I were stained yellow, and collagen type III areas were stained green in the polarized light detection. ( C , D ) Representative images of collagen type I and collagen type III immunohistochemical-stained transverse cardiac sections in offspring. ( E ) Quantification of total fibrosis expressed as a percentage of tissue area by Masson’s trichrome staining ( n = 5). ( F , G ) Collagen type I and collagen type III expression score (H-score) in transverse cardiac sections using immunohistochemical staining ( n = 3). Bar = 20 μm. *** p < 0.001, ** p < 0.01, * p < 0.05.

Article Snippet: Subsequently, the sections were incubated in a picrosirius red staining solution (ab246832, Abcam, Boston, MA, USA) for one hour.

Techniques: Staining, Immunohistochemical staining, Expressing

Journal: bioRxiv

Article Title: GLP-1 Receptor Agonist Improves Metabolic Disease in a Pre-clinical Model of Lipodystrophy

doi: 10.1101/2023.09.01.555852

Figure Lengend Snippet: Male and female wild-type (WT) and seipin knockout (SKO) mice were intraperitoneally injected once daily with phosphate-buffered saline (PBS) or 0.2 mg/kg liraglutide (Lira) for 14 days. ( A ) Liver weight normalised to mice body weight. ( B ) Levels of hepatic triglycerides. ( C, D ) Representative images of hematoxylin and eosin (H&E) and picrosirius red-stained paraffin-embedded liver sections along with representative photographs of macroscopic liver appearance in female ( C ) and male ( D ) mice. Bars denote 200 µm. ( E, F ) Expression levels of a panel of fibrosis markers genes in WT mice and SKO mice treated with PBS or liraglutide. Gene expression was normalised to three reference genes ( Hprt, Nono and Ywhaz ) (female n= 6-7, male n= 8-10). Data are presented as mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001 (PBS versus liraglutide within same genotype); # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001 (WT (PBS) versus SKO (PBS)).

Article Snippet: Liver tissue and pancreata were fixed in 10% neutral buffered formalin, paraffin-embedded and 5 µm-thick sections were stained with hematoxylin and eosin (H&E) or picrosirius red solution (Abcam #ab150681) as in [ ].

Techniques: Knock-Out, Injection, Saline, Staining, Expressing

Journal: Materials Today Bio

Article Title: In situ forming biomaterials as muscle void fillers for the provisional treatment of volumetric muscle loss injuries

doi: 10.1016/j.mtbio.2023.100781

Figure Lengend Snippet: (A) Representative cross sections of H&E and picrosirius red stained tissue 1 day and 28 days after MVF implantation (scale = 500 μm). (B) Rectangularity measurements of implanted MVF. (C) Representative close-up image from WGA-stained muscles (scale = 50 μm). (D) Muscle fiber count of muscles with MVF implantation 1 day and 28 days after implantation. Dotted line and shaded region represents the mean ± SD for fiber number from healthy tibialis anterior muscles. (E) Kernel density estimations of the distribution of minimum Feret diameters 1 and 28 days after MVF implantation. Data in panels B and D were analyzed first via a 2-way ANOVA followed by Holm-Sidak post hoc test. Median data in panel E were analyzed via Kruskal-Wallis test. Post hoc p-values less than 0.05 are listed for all comparisons. Data represented as mean ± SD. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Picrosirius red (PSR) staining was performed with PSR kit (Abcam, ab150681) per the manufacturer's protocol.

Techniques: Staining, Muscles

Journal: Materials Today Bio

Article Title: In situ forming biomaterials as muscle void fillers for the provisional treatment of volumetric muscle loss injuries

doi: 10.1016/j.mtbio.2023.100781

Figure Lengend Snippet: (A) Color deconvolution of picrosirius red stained sections to isolate stained collagen. Zones of 25 μm thickness were made radiating out from the MVF-muscle border, pseudo-colored, and percent area of zone that consisted of collagen was quantified. Graph's dotted line and shaded region represents the mean ± SD for area of collagen in a healthy tibialis anterior muscle. *p < 0.05 for silicone vs PVA and silicone vs HA + PEG. (Scale = 500 μm) (B) Nuclei stained with DAPI and zones of 25 μm thickness were pseudo-colored and percent area of zone that consisted of positive DAPI signal was quantified (Scale = 50 μm). (C) Pan-macrophage marker CD68 was stained to look at cellular infiltrate. Analysis of total CD68 signal in cross section was quantified (Scale = 50 μm). All data were analyzed first via a 1 or 2-way ANOVA followed by Holm-Sidak post hoc test. Post hoc p-values less than 0.05 are listed for all comparisons. Data represented as mean ± SD. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Picrosirius red (PSR) staining was performed with PSR kit (Abcam, ab150681) per the manufacturer's protocol.

Techniques: Staining, Marker